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NeuroMab Utility in Model Organisms (zebrafish, Xenopus, chicken, barn owl)

Zebrafish, Xenopus brain

Utility determined by side-by-side immunoblots versus mammalian brain (typically rat), zebrafish and Xenopus frog brain, or by immunofluorescence staining of 2 and 4 day post-fertilization zebrafish. Many thanks to Martin Meyer (Stanford) and Jerry Thomsen (Stony Brook) for fish and frog samples for immunoblots, and to Bruce Draper (UC Davis) for immunofluorescence staining. Criteria for immunoblots are monospecific reaction against a band similar in molecular characteristics to that in rat. 


Monospecific signal in zebrafish and/or Xenopus by immunoblot
CASK (K56A/50) 
Kv1.1 (K20/78) (fish only)
Kv1.2 (K14/16) 
Kv4.3 (K75/41) (frog only)
KvB2 (K17/70) (fish only)
Pan-MAGUK (K28/86)
Slo1 (L6/60) (fish only)

No signal in either fish or frog by immunoblot
Kv1.4 (K13/31) 
Kv2.1 (K89/34) 
Kv4.2 (K57/1) 
Nav1.2 (K69/3)
PSD95 (K28/43)

Specific immunofluorescence immunolabeling in zebrafish
KvB1.1 (K9/40)
Kv1.2 (K14/16)
pan-beta (K40/17)
KvB1.2 (K47/42)
Pick1 (L20/8)
Kv1.3 (L23/27)
CavB2 (N8B/1)
Mortalin/GRP75 (N52A/42)
SALM2 (N52B/27)
mGluR1/5 (N75/33)
Aldh1L1 (N103/39)
SNAT1 (N104/32)
pan-QKI (N147/6)

 

Avian brain

Utility determined by side-by-side immunoblots versus mammalian brain (typically rat), chicken and barn owl brain. Many thanks to Jessie Whitfield and Dr. Will Debello (UC Davis) for providing data. Criteria are monospecific reaction against an immunoblot band similar in molecular characteristics to that in rat.

Monospecific signal in both chicken and barn owl
Ataxin1 (N76/8)
Cask (K56A/50)
Caspr (K65/35)
Chapsyn-110 (N18/30)
GABAB R2 (N81/2)
Git1 (N39B/8)
GluR2 (L21/32)
HCN2 (N71/37)
IP3R (L24/18)
KChIP1 (K55/7)
KvB1.2 (K47/42)
Kv3.1b (N16b/8)
mGluR1/5 (N75/33)
Mortalin/GRP75 (N52A/42)
NGL-1 (N49A/21)
NR2B (N59/20, N59/36)
pan-MAGUK (K28/86)
pan-Shank (N23B/49)
Pink1 (N4/15)
Salm2 (N52B/27)
SAP102 (N19/2)
Shank2 (N23B/6)
VGlut2 (N29/29)
Wave1/Wasp (K91/36)
Zinedin (K88/64)

No signal in either chicken and barn owl
Adam22 (N57/2)
Cav3.2 (N55/10)
CavB1 (N7/18)
CavB2 (N8B/1)
HCN1 (N70/28)
Kv1.1 (K20/78)
Kv2.1 (K89/41)
Kv4.2 (K57/1)
Kv4.3 (K75/41)
Neurofascin (L11A/41)
SAP97 (K64/15)
SH3GL1 (K51/1)
VGlut1 (N28/9)




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