Please cite the "UC Davis/NIH NeuroMab Facility" as the source of NeuroMabs. Also, please include the complete clone number (e.g., N52A/42) to avoid ambiguity.
A $20 price increase went into effect July 1, 2011. This reflects a requirement from NIH for partial end user support of costs associated with the generation and characterization of future NeuroMabs. Note that the price per ml of tissue culture supernatant, or of 20 micrograms of pure IgG now will be $15, or 15-20% the average price of commercial sources.
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NeuroMab Preparations
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Educational or Non Profit
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For-Profit
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TC Supernatant
(approx. 20 ug/ml IgG)
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Purified IgG
(approx. 1 mg/ml IgG)
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Our catalog has the most up-to-date information on new NeuroMabs, including Production and QC status and newly released catalog #'s, so please use information from there to order NeuroMabs from Antibodies Incorporated.
All NeuroMabs are mouse monoclonal antibodies secreted from hybridoma cells grown either in vitro or in vivo. The different forms are:
TC supernatant: conditioned bovine serum-containing media harvested from hybridomas cultured in vitro. Typically contains 10-50 micrograms/ml mouse monoclonal IgG plus bovine IgG and other bovine serum components.
Recommended working dilutions for TC supernatants are 1:2-1:20 for immunoperoxidase-based immunohistochemistry, and 1:2-1:10 for immunofluorescence-based immunohistochemistry and immunocytochemistry, and immunoblotting. These recommended working dilutions are meant to be a starting point for optimization based on your specific application.
Purified IgG: Purified from hybridoma cells grown in vivo. Predominantly monoclonal IgG but may contain trace amounts of other mouse IgG.
Recommended working concentrations for purified IgGs are 0.1-1 ug/ml for immunoperoxidase-based immunohistochemistry and immunoblotting, and 1-10 ug/ml for immunofluorescence-based immunohistochemistry and immunocytochemistry. These recommended working concentrations are meant to be a starting point for optimization based on your specific application.
A useful general reference for this and related information is Harlow, E. and Lane, D. 1988. Antibodies: A Laboratory Manual , Cold Spring Harbor Laboratory Press