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Welcome to NeuroMab!

The UC Davis/NIH NeuroMab Facility was established in 2004 to generate mouse monoclonal antibodies (mAbs) extensively validated for use in mammalian brain, as funded by NIH awards U24 NS050606 (2005-2014) and R24 NS092991 (2015-2019).

The UC Davis/NIH NeuroMab Facility does not distribute mAbs. UC Davis has agreements with various distributors who produce and distribute mAbs from NeuroMab-provided hybridoma cells.  These mAbs are available from numerous sources at many different price points. Please search the internet for distributors providing specific mAbs using the term "antibody" and the clone number (e.g., "antibody K28/43"). 

The sequences of the heavy and light chain variable domains of most NeuroMabs are now available on the NeuroMabSeq website here.  These sequences were obtained by high-throughput sequencing of hybridoma-derived RNA as funded by BRAIN Initiative award NIH U24 NS109113.

For those who wish to produce mAbs at low cost NeuroMab hybridomas are now available from the UC Davis MMRRC

Plasmids encoding recombinant mAbs are now available from Addgene.  For background information on NeuroMabs that have been cloned and expressed in recombinant form, see Andrews et al., eLife 8:e43322. PubMed.  A related set of plasmids encoding highly validated camelid nanobodies are also available from Addgene.  For background information on these nanobodies, see Dong et al., eLife 8:e48750. PubMed.

NeuroMabs are validated for biochemical and immunohistochemical applications in mammalian brain samples, including immunoblotting, immunohistochemistry and array tomography. For perspectives on application-specific antibody validation see Taussig et al., New Biotech 45:1, 2018. PubMed.

For information on NeuroMab validation see Gong et al., New Biotech 33:551, 2016. PubMed. See detailed sample preparation and labeling protocols used for NeuroMab monoclonal antibody validation.

See publications list for peer-reviewed papers showing data obtained with NeuroMabs, now over >3400 publications!

 

Immunoblot against membranes from postnatal day 8 wild-type (wt) and ADAM22-knockout (ko) mice probed with N46/30 (left, ADAM22-cytoplasmic) and N57/2 (right, ADAM22-extracellular). Data courtesy of Dies Meijer (Erasmus University Medical Center). Immunofluorescence staining of cultured rat hippocampal neurons with K28/43 (green, PSD-95) and K57/1 (red, Kv4.2) Immunofluorescence staining of adult wild-type (WT, top) and Kv4.2 knockout (Kv4.2-/-, bottom) mouse hippocampus with K57/1 (red, Kv4.2) and Kv2.1 rabbit polyclonal (green) Immunoblot against brain membranes from adult rat (RBM) and adult wild-type (MBM-WT) and lethargic (MBM-lh/lh) mice probed with N10/7 (left, Cavbeta4) and K89/41 (right, Kv2.1). Lethargic mice courtesy of Jeffrey Noebels (Baylor College of Medicine). Immunoblot against membranes from adult rat brain (RBM) and hippocampi from adult wild-type (MHM-WT) and Chapsyn-110 knockout (MHM-Chapsyin-110 KO) mice probed with N18/30 (Chapsyn-110). Mouse samples courtesy of Richard Huganir (Johns Hopkins University, Howard Hughes Medical Institute) Immunofluorescence staining of hippocampal CA3 of adult wild-type (WT, left top) and Slo1 knockout (KO, left bottom) mice with L6/60 (red, Slo1) and Kv2.1 rabbit polyclonal (green). Immunoblot against brain membranes from adult rat (RBM) and adult WT and Slo1 KO mice probed with L6/60 (right, Slo1)

 

 

 

 

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