NeuroMab Utility for Array Tomography
Array tomography (AT) validation was performed in part under the auspices of NIH award R01 NS092474 "Synaptomes of Mouse and Man", PI Stephen J. Smith and NIH award U24 NS109113 "Recombinant Immunolabels for Nanoprecise Brain Mapping Across Scales", PI James S. Trimmer.
Mouse and human brain AT immunofluorescence via LRWhite protocol references: Micheva et al., 2010. PubMed 21092855, 21041404
Mouse brain AT conjugate immunofluorescence / electron microscopy via Lowicryl protocol reference: Collman et al., 2015. PubMed 25855189
For further information see Micheva et al., 2023. PubMed 37945352.
This table is also available as a downloadable CSV file.
| Target | Clone | Isotype | Mouse LRWhite | Mouse Lowicryl | Human LRWhite | |
|---|---|---|---|---|---|---|
| Bassoon | L124/59 | IgG2b | PASS | FAIL | ND | |
| Calbindin | L109/39 | IgG2b | PASS | PASS | ND | |
| Calbindin | L109/57 | IgG1 | PASS | PASS | PASS | |
| Calretinin | L122/6 | IgG2b | PASS | ND | ND | |
| Calretinin | L122/68 | IgG2a | PASS | ND | ND | |
| CASK | K56A/50 | IgG1 | PASS | FAIL | ND | |
| CASPR/Neurexin IV | K65/35 | IgG1 | PASS | PASS | ND | |
| Cav3.1 | N178A/9 | IgG1 | PASS | ND | ND | |
| Collybistin | L120/12 | IgG1 | PASS | PASS | ND | |
| DLX1 | L43/40 | IgG2a | FAIL | ND | ND | |
| GABAARAlpha1 | N95/35 | IgG2a | PASS | FAIL | PASS | |
| GABAARBeta1 | N96/55 | IgG1 | PASS | ND | ND | |
| GABAARBeta3 | N87/25 | IgG1 | PASS | ND | ND | |
| GABABR1 | N93A/49 | IgG1 | PASS | ND | ND | |
| GAD65/67 | L127/12 | IgG2a | PASS | PASS | PASS | |
| GAD67 | L127/8 | IgG2b | PASS | PASS | FAIL | |
| Gephyrin | L106/4 | IgG1 | PASS | PASS | PASS | |
| Gephyrin | L106/83 | IgG2b | PASS | PASS | PASS | |
| Gephyrin | L106/93 | IgG2a | PASS | PASS | PASS | |
| GFP | N86/8 | IgG2a | PASS | ND | NA | |
| GluN2A/NR2A | N327/95 | IgG2a | PASS | FAIL | PASS | |
| GluN2B/NR2B | N59/20 | IgG2a | FAIL | ND | ND | |
| GluN2B/NR2B | N59/36 | IgG2b | PASS | PASS | PASS | |
| GluR2 | L21/32 | IgG1 | PASS | FAIL | ND | |
| Homer1L | L113/13 | IgG1 | PASS | ND | ND | |
| Homer1L/S | L113/130 | IgG1 | PASS | PASS | ND | |
| IP3 Receptor Type1 | L24/18 | IgG1 | PASS | ND | ND | |
| IRSp53/BAIAP2 | L117/1 | IgG1 | PASS | PASS | ND | |
| KChIP1 | K55/7 | IgG1 | PASS | ND | ND | |
| KCNT1/Slo2.2/Slack | N3/26 | IgG1 | FAIL | ND | ND | |
| Kv1.1 (external) | K36/15 | IgG2b | PASS | PASS | ND | |
| Kv1.2 | K14/16 | IgG2b | PASS | PASS | ND | |
| Kv2.1 | K89/34 | IgG1 | PASS | PASS | ND | |
| Kv4.2 | K57/1 | IgG1 | PASS | ND | ND | |
| Kv7.2/KCNQ2 | N26A/23 | IgG1 | PASS | ND | ND | |
| LRRTM2 | N209C/35 | IgG1 | PASS | ND | ND | |
| mGluR1/5 | N75/33 | IgG1 | FAIL | ND | ND | |
| Miro2 | N384/63 | IgG1 | PASS | PASS | ND | |
| Nav1.1 | K74/71 | IgG1 | ND | FAIL | ND | |
| Nav1.6 | K87A/10 | IgG1 | FAIL | FAIL | ND | |
| Neurabin-1/Ppp1r9a | L129/93 | IgG1 | PASS | PASS | ND | |
| Neuropeptide Y | L115/13 | IgG2a | PASS | PASS | PASS | |
| nNOS/NOS1 | L121/25 | IgG2b | PASS | PASS | PASS | |
| Pan-MAGUK | K28/86 | IgG1 | PASS | ND | ND | |
| Pan-QKI | N147/6 | IgG2b | PASS | PASS | ND | |
| Pan-SAPAP | N127/31 | IgG2b | PASS | PASS | ND | |
| Pan-Shank | N23B/49 | IgG1 | FAIL | ND | ND | |
| Parvalbumin | L114/81 | IgG1 | PASS | FAIL | FAIL | |
| PSD-93/Chapsyn-110 | N18/30 | IgG1 | PASS | PASS | ND | |
| PSD95 | K28/43 | IgG2a | PASS | PASS | PASS | |
| PSD95 | K28/74 | IgG1 | PASS | ND | PASS | |
| SAP102 | N19/2 | IgG1 | PASS | PASS | ND | |
| SAP97 | K64/15 | IgG1 | PASS | ND | ND | |
| Synaptotagmin-12 | N277/7 | IgG1 | PASS | ND | ND | |
| Syncam1 | L45/30 | IgG1 | FAIL | ND | ND | |
| TARPGamma2/Stargazin | N245/1 | IgG2a | PASS | ND | ND | |
| VAChT | N6/38 | IgG1 | FAIL | ND | ND | |
| VDAC1 | N152B/23 | IgG2a | PASS | FAIL | ND | |
| VGAT | L118/66 | IgG2a | PASS | PASS | ND | |
| VGlut1 | N28/9 | IgG1 | PASS | ND | PASS | |
| VGlut2 | N29/29 | IgG1 | PASS | FAIL | FAIL | |
| WAVE1/SCAR | K91/36 | IgG1 | PASS | ND | ND |